This work is a contribution to the Psychodidae fauna of Belize, in the subfamilies Bruchomyiinae and Phlebotominae. Nemapalpus mopani De León was recorded and represents the first record of a Bruchomyiinae in Belize. The subfamily Phlebotominae is represented by 22 nominated species, but Lutzomyia serrana (Damasceno & Arouck) and L. aclydifera (Fairchild & Hertig) are recorded for the first time in this country. Lutzomyia manciola, a new species, is described, bringing the number of phlebotomine species in Belize to 25, including the three previously recorded species of Brumptomyia França & Parrot. Keys for the identification of males and females of all 22 Belizean species of Lutzomyia are included.